Synthesising Peptides

Hydrolysing Polypeptides


  1. Draw a pencil line approximately 1cm from the bottom of the paper. Mark a cross and produce a concentrated spot of the compounds to be used by repeated application followed by drying.
  2. Mark a reference spot of aspartic acid, and phenylalanine.
  3. Mark a spot of the product from the hydrolysis of aspartame.
  4. Place in chromatography tank; ensure that the spots are above the solvent (butan-1-ol and ethanoic acid).
  5. Cover with lid.
  6. Allow time for the solvent to run up the chromatography plate, so it reaches just below the end of the paper.
  7. Use locating agent to find spots (ninhydrin), and then heat using a hair dryer.
  8. Compare the products from the hydrolysis of aspartame with the reference compounds.

    Useful books for revision

    Revise A2 Chemistry for Salters (OCR A Level Chemistry B)
    Salters (OCR) Revise A2 Chemistry